SEPARATION CHROMATOGRAPHIQUE DES SULFATES ET DES GLYCURONIDES DE STEROIDES URINAIRES

Abstract

A method, using adsorption on alumina and elution by hydrated butanol for the separation of the steroid glycuronides and sulfates, was applied to study the urines of a 2-year-old girl with adrenal carcinoma. Extracts obtained at.pH 11 and pH 3.5 were chromatographed separately. Extracts at pH 11 : A study of steroid liberated by hydrolysis showed that in the sulfate fraction 95% of the 17-ketosteriods are dehydroepiandrosterone. There is also a small quantity of epiandrosterone, androsterone and etiocholanolone. In the glucuronide fraction, 17-ketosteroids are composed mainly of androsterone and etiocholanolone. Neither dehydroepiandrosterone nor epiandrosterone was found. Extracts at pH 3.5: They showed only traces of sulfoconjugates. In the glucuronide fraction 17-ketosteroids proved to be only 11-oxy-17-ketosteroids. The presence of C19 or C21 alcoholic steroids is pointed out.