The rapid determination of diazinon and its oxygen analogue in animal tissues by gas chromatography

Abstract

A rapid method for the simultaneous gas-chromatographic determination of diazinon and its oxygen analogue (diazoxon) in blood, fat, liver, muscle and brain is described. The use of a selective thermionic phosphorus detector makes clean-up unnecessary. Preparation of the sample before injection consists in trituration with sand and sodium sulphate, elution with methanol or ether and concentration. Quantitative measurements are made by comparison with an internal standard. The method is satisfactory for the determination of 0·05 p.p.m. of diazinon and 0·2 p.p.m. of diazoxon in a 0·1-g sample.