Incorporation of 6 mg/liter chloromycetin into the germination medium does not appreciably affect the number of spores that germinate and develop into visible colonies. This concentration of chloromycetin inhibits many of the bacteria normally found in sporophores of C. gigantea. Incubation temperatures of 24-26[degree]C are most suitable for germinating basidiospores of C. gigantea. The number of spores inoculated into each Petri dish affects the germination per million. Although this figure varies from 1 sporophore to another and appears to decrease as the germination per million increases, from present data a spore concentration of 1.0 - 5.0 x 106/Petri dish usually results in the greatest germination per million. Storage temperatures of 12[degree]C or -18[degree]C appear to maintain with greater consistency the germination per million as opposed to storage at room temperature. This also varies somewhat between different sporophores. An increase of the ger- -mination per million for spores from some sporophores after increased storage time tends to imply that an after-ripening period is necessary for spores of C. gigantea. Present data suggest that the germination per million decreases when spores have been stored in excess of 1-2 years. Greater testing of this factor is necessary.