Ultrastructural localization of alpha-OH acid oxidase in peroxisomes with the CeCl3 technique.
Open Access
- 1 September 1980
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 28 (9), 1025-1028
- https://doi.org/10.1177/28.9.6997367
Abstract
Alpha-OH acid oxidase activity was demonstrated in peroxisomes of glutaraldehyde-fixed tissues using the CeCl3 cytochemical method. For kidney, alpha-OH butyrate or alpha-OH valerate were used as substrates. These substrates gave much less reaction product in liver peroxisomes. Liver peroxisomes were more reactive with glycolate as substrate. Glycolate gave little or no reaction product in kidney peroxisomes. alpha-keto-glutarate inhibited activity of the enzyme with alpha-OH butyrate as substrate.This publication has 4 references indexed in Scilit:
- Ultrastructural localization of D-amino acid oxidase in microperoxisomes of the rat nervous system.Journal of Histochemistry & Cytochemistry, 1979
- Microperoxisomes in the central nervous system of the postnatal ratBrain Research, 1978
- The cytochemical demonstration of catalase and D-amino acid oxidase in the microbodies of teleost kidney cellsJournal of Molecular Histology, 1977
- CYTOCHEMISTRY AND ELECTRON MICROSCOPYThe Journal of cell biology, 1963