Comparison of Tetracycline Action on Staphylococcus aureus and Escherichia coli by Microbial Kinetics
Open Access
- 1 August 1976
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 10 (2), 223-228
- https://doi.org/10.1128/aac.10.2.223
Abstract
Cultures of tetracycline-treated Staphylococcus aureus exhibited monophasic steady-state growth curves similar to that observed for tetracycline-treated Escherichia coli . Apparent growth rate constants of the respective drug-treated cultures showed the same formal dependence on drug concentration, which was linear at a low concentration but asymptotically approached zero at higher concentration levels and implied the saturation of a limited number of receptor sites engaged in microbial protein synthesis. The relative potency of tetracycline action of S. aureus/E. coli was 6.50:1 at 37.5°C and pH 7.05. This is attributed to relative differences in drug permeation and/or binding affinity for biophase receptors in the respective organisms. It is concluded from kinetic dependencies of growth inhibition of the cultures that tetracycline has the same mode of action on S. aureus and E. coli . It is bacteriostatic at concentrations below the minimal inhibitory concentration level but bactericidal at the higher concentration levels.Keywords
This publication has 17 references indexed in Scilit:
- Microbial Kinetics of Drug Action against Gram-Positive and Gram-Negative Organisms I: Effect of Lincomycin on Staphylococcus aureus and Escherichia coliJournal of Pharmaceutical Sciences, 1974
- Effect of Novobiocin and Its Combination with Tetracycline, Chloramphenicol, Erythromycin, and Lincomycin on the Microbial Generation of Escherichia coliAntimicrobial Agents and Chemotherapy, 1973
- Microbial Kinetics and Dependencies of Individual and Combined Antibiotic Inhibitors of Protein BiosynthesisAntimicrobial Agents and Chemotherapy, 1973
- Structure-Activity Relationships of Tetracyclines I: Inhibition of Cell Division and Protein and Nucleic Acid Syntheses in Escherichia coli WJournal of Pharmaceutical Sciences, 1971
- Mechanism of Tetracycline Resistance in Staphylococcus aureusJournal of General Microbiology, 1970
- Studies on the binding of tetracycline to ribosomesBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
- Inhibition of formylmethionyl-transfer RNA binding to ribosomes by tetracycline.Proceedings of the National Academy of Sciences, 1968
- Bacterial protein synthesis: The effects of antibioticsJournal of Molecular Biology, 1967
- Kinetics and Mechanisms of Action of Antibiotics on Microorganisms VJournal of Pharmaceutical Sciences, 1966
- Kinetics and Mechanisms of Action of Antibiotics on Microorganisms III.Journal of Pharmaceutical Sciences, 1965