Regional mapping of 22 microclones around the adenomatous polyposis coli (APC) locus on chromosome 5q
- 1 November 1991
- journal article
- Published by Springer Nature in Human Genetics
- Vol. 88 (1), 112-114
- https://doi.org/10.1007/bf00204940
Abstract
A previously described genomic library constructed from microdissected DNA has been used to generate a large number of probes around the adenomatous polyposis coli (APC) gene at 5q22. A total of 202 clones were hybridised directly onto a somatic cell hybrid panel containing two APC-related interstitial deletions. Of 75 microclones that gave clear hybridisation signals, 22 independent clones mapped into the region common to both deletions. In addition, 4/22 of the markers are conserved in rodent DNA. These clones should provide a valuable resource for screening cDNA libraries and cloning the DNA around the APC gene in yeast artificial chromosomes.This publication has 16 references indexed in Scilit:
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