Improved capillary gas-chromatographic-mass spectrometric method for the determination of anabolic steroid and corticosteroid metabolites in horse urine using on-column injection with high-boiling solvents

Abstract

When analysing large numbers of samples containing high-boiling components by capillary column gas chromatography, the analysis time using hexane as solvent in the splitless mode is prohibitive. In order to reduce the analysis time for the steroid TMS and MO-TMS derivatives but yet maintain a solvent effect, the use of solvents with high boiling-points has been investigated. The use of solvents with high boiling-points has now been extended to on-column injection without any loss in resolution or adverse effects on column lifetime.