BIOGENESIS OF RIBOFLAVIN IN GREEN LEAVES

Abstract

V compound was produced non-enzymatically from G compound. The conversion reaction of G compound into V compound proceeded enzymatically in the presence of the enzyme preparation obtained from spinach. The enzymatic formation of V compound from G compound required the presence of molecular oxygen, and was inhibited completely under anaerobic conditions. The enzymatic reaction to form riboflavin from G compound proceeded without molecular oxygen. The stoichiometry of the enzymatic synthesis of riboflavin was established by the reaction under "anaerobic condition." Formation of V compound was inhibited by KCN and KF.