The ATP‐driven primary Na+ pump in subcellular vesicles of Vibrio alginolyticus
- 20 June 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 233 (2), 355-358
- https://doi.org/10.1016/0014-5793(88)80459-9
Abstract
Subcellular vesicles of Vibrio alginolyticus hydrolyse ATP and accumulate Na+ in an ATP-dependent fashion. The Na+ uptake is (i) strongly stimulated by Δψ-discharging agents, i.e., the protonophorous uncoupler CCCP or valinomycin +K+ and (ii) arrested by DCCD at a concentration strongly inhibiting ATP hydrolysis. Lower concentrations of DCCD stimulate the Na+ accumulation supported by ATP hydrolysis as well as by NADH oxidation. It is concluded that there is an electrogenic DCCD-sensitive Na+-ATPase in the cytoplasmic membrane of V. alginolyticus.Keywords
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