Wound Healing: Glycoproteins of Wound Tissue. I. Studies of Hexosamine, Hexose, and Uronic Acids Content.

Abstract

A serial study of uronic acid, hexosamine, and hexose content of wound repair tissue was made utilizing the tissue formed around stainless steel cylinders implanted sub-cutaneously in dogs. The cylinders were removed at various times after implantation for quantitative studies. The uronic acid content was quite low in the 7-day sample and increased until about the 28th day. Hexosamine in considerable excess of the uronic acid was found indicating the presence of polysacchrides or glycoproteins which did not contain uronic acid. Hexosamine was relatively high in early samples and did not change strikingly in later samples. Sugars with the electro-phoretic mobility (in borate buffer) of galactose, mannose, glucose, and uronic acid were found in the hydrolysates of wound repair tissue.