The fractional disappearance rate of the separate isoenzymes of creatine phosphokinase in the dog

Abstract

Separation of creatine phosphokinase (CPK) isoenzymes from canine tissue was accomplished successfully with column chromatography. Intravenous injection of each isoenzyme into six dogs resulted in a mono-exponential loss with differing fractional disappearance rates which averaged 0.65%, 0.86%, and 1.68%/min for the MM, MB, and BB isoenzymes, respectively. The distribution volume also varied: MM averaged 6.7%/body weight, MB 7.8%, and BB 10.4%.