New staining techniques for the Langerhans cell
Open Access
- 1 July 1977
- journal article
- research article
- Published by Medical Journals Sweden AB in Acta Dermato-Venereologica
- Vol. 57 (4), 289-296
- https://doi.org/10.2340/0001555557289296
Abstract
Three new techniques are described for staining the Langerhans cell in whole mounts of fresh human and guinea pig epidermis. These employ paraphenylenediamine, gold sodium thiomalate and cobalt chloride, respectively, and require appropriate epidermal separation with EDTA, ammonium thiocyanate or sodium bromide. Used in conjunction with a modified adenosine triphosphatase stain, these techniques provide greater capability for observing the Langerhans cell in disease states than can be achieved by any single stain. A combined stain with adenosine triphosphate and gold is also described.This publication has 3 references indexed in Scilit:
- Quantitative Studies on the Langerhans Cell Population of Guinea Pig Epidermis**From the Section of Dermatology Mayo Clinic and Mayo Foundation and the Mayo Graduate School of Medicine University of Minnesota, Rochester, Minnesota.Journal of Investigative Dermatology, 1967
- Adenosine triphosphatase activity in neural elements of human epidermisExperimental Cell Research, 1962
- An Electron Microscope Study of Basal Melanocytes and High-Level Clear Cells (Langerhans Cells) in Vitiligo**From the Chester Beatty Research Institute, Royal Cancer Hospital, London, S.W. 3, and the Departments of Anatomy, and Dermatology, St. Mary's Hospital Medical School (University of London) London, W. 2, England.Journal of Investigative Dermatology, 1961