Use of a Low‐Ionic‐Strength Medium in Manual Tests for Antibody Detection
- 8 July 1976
- journal article
- research article
- Published by Wiley in Transfusion
- Vol. 16 (4), 291-296
- https://doi.org/10.1046/j.1537-2995.1976.16476247048.x
Abstract
The present observations confirm the value of suspending [human] red blood cells in a low-ionic-strength medium in the 1st stage of the indirect antiglobulin test, i.e., during the period of incubation with antibody-containing serum. The main advantage of this procedure is to shorten the time of incubation. In this respect, a low-ionic-strength medium appears to be superior to albumin as a suspending medium for the red blood cells. A further advantage is to increase the uptake of certain antibodies; this effect was pronounced with selected Rh antibodies believed to be of low affinity.This publication has 9 references indexed in Scilit:
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