Calcium‐dependence of chromogranin A‐catecholamine interaction
- 7 November 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 239 (2), 203-206
- https://doi.org/10.1016/0014-5793(88)80917-7
Abstract
Major components of the secretory organelle of bovine adrenal medullary cells, the chromaffin vesicles, are the acidic protein chromogranin A, catecholamines and Ca2+. The binding of Ca2+ to chromogranin A has been established. To study the interaction between chromogranin A and catecholamines and its dependence on Ca2+ we immobilized chromogranin A to a newly raised monoclonal antibody. It is shown that chromogranin A can bind (i) about 0.5 mol catecholamines per mol in a non-calcium-dependent manner and (ii) about 5 mol per mol in the presence of calcium. These results further support the notion that chromogranin A may act as a secretory granule-condensing proteinKeywords
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