Microfilament distribution in protonemata of the mossCeratodon
- 1 September 1995
- journal article
- research article
- Published by Springer Nature in Protoplasma
- Vol. 189 (3-4), 229-237
- https://doi.org/10.1007/bf01280177
Abstract
Microfilaments were visualized in dark-grown protonemata of the mossCeratodon to assess their possible role in tip growth and gravitropism. The relative effectiveness of rhodamine phalloidin (with or without MBS) and of immunofluorescence (using the C4 antibody) was evaluated for actin localization in the same cell type. Using immunofluorescence, microfilaments were primarily in an axial orientation within the apical cell. However, a more complex network of microfilaments was observed using rhodamine phalloidin after MBS pretreatment, especially when viewed by confocal laser scanning microscopy. This method revealed a rich three dimensional network of fine microfilaments throughout the apical cell, including the extreme apex. Although there were numerous internal microfilaments, peripheral microfilaments were more abundant. No major redistribution of microfilaments was detected after gravistimulation. The combination of MBS, rhodamine phalloidin, and confocal laser scanning microscopy preserves and reveals microfilaments remarkably well and documents perhaps the most extensive F-actin network visualized to date in any tip-growing cell.Keywords
This publication has 30 references indexed in Scilit:
- The formation of aplastidic abscission (tmema) cells and protonemal disruption in the mossBryum tenuisetum Limpr. is associated with transverse arrays of microtubules and microfilamentsProtoplasma, 1993
- The dynamic behavior of cytoplasmic F-actin in growing hyphaeProtoplasma, 1993
- The organization of F-actin in root tip cells ofAdiantum capillus veneris throughout the cell cycleProtoplasma, 1992
- Actin filaments line up acrossTradescantia epidermal cells, anticipating wound-induced divison planesProtoplasma, 1990
- Microtubule distribution in gravitropic protonemata of the mossCeratodonProtoplasma, 1990
- Cortical fine actin filaments in higher plant cells visualized by rhodamine-phalloidin after pretreatment with m-maleimidobenzoyl N-hydroxysuccinimide esterProtoplasma, 1989
- Visualization of actin filament pattern in plant cells without pre-fixation A comparison of differently modified phallotoxinsProtoplasma, 1989
- Microtubules and actin filaments co-localize in pollen tubes ofNicotiana tabacum L. andLilium longiflorum Thunb.Protoplasma, 1989
- A cytokinin-sensitive mutant of the moss,Physcomitrella patens, defective in chloroplast divisionProtoplasma, 1989
- Fluorescence microscopic localization of actin in pollen tubes: Comparison of actin antibody and phalloidin stainingCell Motility, 1989