NUTRITION OF CELLULAR SLIME MOLDS III
Open Access
- 1 December 1963
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 86 (6), 1314-1320
- https://doi.org/10.1128/jb.86.6.1314-1320.1963
Abstract
Hohl, Hans-Rudolf (University of Wisconsin, Madison), and Kenneth B. Raper. Nutrition of cellular slime molds. III. Specific growth requirements of Polysphondylium pallidum. J. Bacteriol. 86:1314–1320. 1963.—Soluble media for the axenic growth of certain strains of Polysphondylium pallidum are described. One of the media, referred to as the complex medium, was composed of 2% tryptose and 4% serum albumin in an inorganic salt solution. Cell densities of up to 107 myxamoebae per ml were obtained. Supplementing this medium with amino acids, vitamins, nucleic acid bases, and glucose resulted in growth of strain Pan-17 up to 3 × 107 cells per ml with a generation time during the log phase of about 4.5 hr. A defined medium was subsequently developed that allowed growth of strain Fr-47 up to 2 × 106 to 5.5 × 106 myxamoebae per ml in the presence of 1% serum albumin. The base medium contained the following essential nutrients: six amino acids (glycine, methionine, lysine, isoleucine, tryptophan, and tyrosine), riboflavine, purines and pyrimidines, and a carbohydrate (dextrose) dissolved in an inorganic salt solution containing trace elements.Keywords
This publication has 24 references indexed in Scilit:
- Albumin Replacement by Fatty Acids in Clonal Growth of Mammalian CellsScience, 1963
- Growth of the Cellular Slime Mold Polysphondylium pallidum in a Simple Nutrient MediumScience, 1963
- The Amino Acid Requirements of the Soil Amoeba, Hartmannella rhysodes Singh*The Journal of Protozoology, 1962
- Influence of serum protein on determination of nutritional requirements of cells in cultureExperimental Cell Research, 1962
- A Defined Medium for Haemophilus influenzae and Haemophilus parainfluenzaeJournal of General Microbiology, 1962
- The Growth of Acanthamoeba sp. in a Chemically Defined Medium519Journal of General Microbiology, 1959
- Permeability studies on the amoebae of the slime mold, dictyostelium mucoroidesJournal of Cellular and Comparative Physiology, 1959
- Nutritional and Related Biological Studies on the Free-Living Soil Amoeba, Hartmannella rhysodesJournal of General Microbiology, 1959
- NITROGEN METABOLISM OF THE SLIME MOLD DICTYOSTELIUM DISCOIDEUM DURING GROWTH AND MORPHOGENESISThe Biological Bulletin, 1954
- A Preliminary Enumeration of the SorophoreæProceedings of the American Academy of Arts and Sciences, 1901