Production of Antibodies to Carcinoembryonic Antigen in Tolerant Rabbits

Abstract

Antisera reacting with carcinoembryonic antigens isolated from metas-tases of colonic cancer were produced in mature rabbits injected at birth with heterologous proteins. The antisera were examined by the agarose gel diffusion, immunoelectrophoresis, counterimmunoelectrophoresis, and radioimmunoassay techniques. The anti-CEA antisera did not need absorption with extracts of normal liver and colon to remove antibodies to them and were interchangeable with goat anti-CEA antisera for use in assay systems for circulating CEA. The immunizing procedure using aluminum hydroxide gel as an adjuvant is described.