Studies of ovulation in the perfused ovary of the fowl (Gallus domesticus)
- 1 July 1987
- journal article
- research article
- Published by Bioscientifica in Reproduction
- Vol. 80 (2), 411-416
- https://doi.org/10.1530/jrf.0.0800411
Abstract
A system was developed for the in-vitro perfusion of the fowl ovary. The ovaries were isolated 16-18 h before expected ovulation of the first follicle of a clutch sequence and perfused at 41.degree.C with Eagle''s culture medium containing L-thyroxine and insulin. The efferent perfusion pressure was maintained at 30-40 mmHg. This model was used to investigate the mechanism of ovulation. Addition of LH (1 U) to the perfusate induced ovulation (46%) but LH (1 U) + FSH (1 U) was more effective (88%; P < 0.05). Progesterone at 100 .mu.g alone also induced ovulation (80%). Clomiphene prevented gonadotrophin-induced ovulation. These results suggest that progesterone may act directly on the ovary as a final hormone to induce ovulation in the domestic fowl.This publication has 14 references indexed in Scilit:
- Influence of Follicular Maturation on Progesterone Release in Chicken Granulosa Cells in Response to Turkey and Ovine GonadotropinsBiology of Reproduction, 1981
- Effects of steroid blockers on LH-induced ovulation in the domestic fowl, Gallus domesticusReproduction, 1981
- Effect of ovine LH on the progesterone content of the granulosa cells in preovulatory follicles of the domestic fowl (Gallus domesticus)Reproduction, 1980
- EFFECT OF LUTEINIZING HORMONE ON PROGESTERONE SECRETION IN VITRO BY THE GRANULOSA CELLS OF THE DOMESTIC FOWL (GALLUS DOMESTICUS)Journal of Endocrinology, 1980
- CONTROL OF THE PREOVULATORY SURGE OF LUTEINIZING HORMONE IN THE HEN (GALLUS DOMESTICUS): THE ROLE OF PROGESTERONE AND ANDROGENSJournal of Endocrinology, 1978
- THE PLASMA CONCENTRATIONS OF TESTOSTERONE AND LH DURING THE OVULATION CYCLE OF THE HEN (GALLUS DOMESTICUS)Acta Endocrinologica, 1977
- THE INTERRELATIONSHIP BETWEEN PROGESTERONE AND LUTEINIZING HORMONE DURING THE OVULATION CYCLE OF THE HEN (GALLUS DOMESTICUS)Journal of Endocrinology, 1976
- EFFECTS OF ANDROGENS, OESTROGENS AND DEOXYCORTICOSTERONE ACETATE ON PLASMA CONCENTRATIONS OF LUTEINIZING HORMONE IN LAYING HENSJournal of Endocrinology, 1976
- The effects of progesterone on oviposition and ovulation in the domestic fowl (Gallus domesticus)British Poultry Science, 1976
- The blood‐vascular system of the chicken ovaryJournal of Anatomy, 1949