Isolation and characterization of a mammalian gene encoding a high-affinity cAMP phosphodiesterase.
- 1 May 1989
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 86 (10), 3599-3603
- https://doi.org/10.1073/pnas.86.10.3599
Abstract
A rat brain cDNA library has been constructed in a Saccharomyces cerevisiae expression vector and used to isolate genes that can function in yeast to suppress the phenotypic effects of RAS2val19, a mutant form of the RAS2 gene analogous to an oncogenic mutant of the human HRAS gene. One cDNA, DPD, was cloned and its genetic and biochemical properties were characterized. A DPD production would share 80% amino acid sequence identity with the Drosophila melanogaster dunce-encoded protein over an extended region. We have shown that the DPD protein is a high-affinity cAMP-specific phosphodiesterase.This publication has 34 references indexed in Scilit:
- Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA PolymeraseScience, 1988
- Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2Nature, 1987
- RAM, a gene of yeast required for a functional modification of RAS proteins and for production of mating pheromone a-factorCell, 1986
- In yeast, RAS proteins are controlling elements of adenylate cyclaseCell, 1985
- Functional homology of mammalian and yeast RAS genesCell, 1985
- Genes in S. cerevisiae encoding proteins with domains homologous to the mammalian ras proteinsCell, 1984
- Studies on transformation of Escherichia coli with plasmidsJournal of Molecular Biology, 1983
- Dunce mutants of Drosophila melanogaster: mutants defective in the cyclic AMP phosphodiesterase enzyme system.The Journal of cell biology, 1981
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- Calcium-dependent bacteriophage DNA infectionJournal of Molecular Biology, 1970