Buoyancy regulation in phosphate-limited cultures of Microcystis aeruginosa

Abstract

Buoyancy regulation was studied in P-limited continuous cultures of Microcystis aeruginosa grown on light-dark cycles of 8–16 h. Gas-vesicle content did not vary systematically over a range of dilution rates form 0.004 to 0.015 h⁻¹. A reduction in irradiance did not cause a significant change in gas-vesicle content. The proportion of floating cells decreased during the photoperiod and increased during the dark period. At three dilution rates, parallel cultures were grown at growth-saturating irradiance and at a lower irradiance. The cultures at low irradiance had a higher proportion of floating cells and a smaller decrease in buoyancy during the light period. The buoyancy losses were not due to destruction of gas vesicles but, rather, to the accumulation of heavy substances. However, measured increases in polysaccharide ballast accounted for only 60% of the required ballast. The molecule(s) which comprised the remainder of the ballast are unknown. Upon relief of phosphate limitation, P-limited cultures increased their buoyancy when incubated in the dark or light. Buoyancy increases in the dark were correlated with a decrease in polysaccharide content, whereas there was an increase in gas vesicle content in the light.