Regulation of amino acid transport in chicken embryo fibroblasts by purified multiplication-stimulating activity (MSA)
- 1 January 1980
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 102 (1), 55-62
- https://doi.org/10.1002/jcp.1041020109
Abstract
Enhanced amino acid transport is observed when quiescent cultures of chicken embryo fibroblasts are stimulated to proliferate by the addition of purified multiplication‐stimulating activity (MSA). This increase in amino acid transport is an early event occuring prior to the onset of DNA synthesis in stimulated cells. Results indicate that the changes in transport activity, as measured by α‐aminoisobutyric acid (AIB) uptake, are due to stimulation of only the Na+‐dependent A transport system. There is little or no change in the activities of transport systems ASC, L, or Ly+ upon exposure to MSA. A kinetic analysis shows this increased activity is due to a change in Vmax while Km remains unaltered. Continuous exposure to the stimulus is required to maintain the increased level of transport activity and the presence of inhibitors of RNA and protein synthesis significantly inhibits the response. Results also indicate that a similar specific increase in the A transport system is initiated when RSV tsNY68 infected cells are shifted to the permissive temperature. It appears that the A system of mediation is emerging as a strategic regulatory site for cell function.This publication has 30 references indexed in Scilit:
- Regulation of the proliferative response in Rous sarcoma virus transformed chicken embryo fibroblasts by serum and multiplication-stimulating activity (MSA)Journal of Cellular Physiology, 1979
- Polyamines and the stimulation of DNA synthesis in chicken embryo fibroblasts by purified multiplication-stimulating activityLife Sciences, 1978
- Large scale purification and further characterization of a rat liver cell conditioned medium multiplication stimulating activityJournal of Cellular Physiology, 1977
- Control of the uptake of amino acids by serum in chick embryo cells, untransformed or transformed with rous sarcoma virusThe Journal of Membrane Biology, 1976
- Precautions that we are observing one transport system unobscured by the contributions of othersJournal of Cellular Physiology, 1976
- α-Aminoisobutyric Acid Transport into Human Glia and Glioma Cells in CultureJournal of Cellular Physiology, 1976
- Control of growth of mammalian cells in cell cultureNature, 1975
- Deoxyglucose transport by uninfected, murihe sarcoma virus‐transformed, and murine leukemia virus‐infected mouse cellsJournal of Cellular Physiology, 1973
- Multiplication‐stimulating activity for chicken embryo fibroblasts from rat liver cell conditioned medium: A family of small polypeptidesJournal of Cellular Physiology, 1973
- A partially purified polypeptide fraction from rat liver cell conditioned medium with multiplication‐stimulating activity for embryo fibroblastsJournal of Cellular Physiology, 1973