Simple and rapid method for purification of vinculin from bovine aorta
- 1 December 1990
- journal article
- Published by Springer Nature in Journal of Muscle Research and Cell Motility
- Vol. 11 (6), 465-470
- https://doi.org/10.1007/bf01745214
Abstract
A new rapid purification procedure has been developed for mammalian smooth muscle vinculin. Bovine aorta vinculin has been purified by a two-step procedure employing hydrophobic chromatography on phenyl-Sepharose and gel filtration. This procedure should be particularly useful for purifying vinculin from tissues in which it is present in a relatively low concentration. In addition, the new purification protocol provides highly pure vinculin free from active contaminants which reduce the low-shear viscosity of F-actin solution.Keywords
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