Characterization of Plasmid pRT1 from Pyrococcus sp. Strain JT1
Open Access
- 1 May 2002
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 184 (9), 2561-2566
- https://doi.org/10.1128/jb.184.9.2561-2566.2002
Abstract
We discovered a 3,373-bp plasmid (pRT1) in the hyperthermophilic archaeon Pyrococcus sp. strain JT1. Two major open reading frames were identified, and analysis of the sequence revealed some resemblance to motifs typically found in plasmids that replicate via a rolling-circle mechanism. The presence of single-stranded DNA replication intermediates of pRT1 was detected, confirming this mode of replication.Keywords
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