Abstract
There is an 85,000-dalton protein, called 85K [kilodalton] amorphin, associated with the Z-band of chicken pectoralis muscle myofibrils. This protein was purified and isolated. It is not a structural component of the Z-filaments, since it can be extracted completely without extraction of the Z-filaments. Extraction of 85K amorphin results in loss of specific staining of the Z-band with fluorescence specific anti-85K amorphin. Actinin is the structural component of the Z-filaments, since extraction of .alpha.-actinin is accompanied by loss of the Z-filament structure.