THE EFFECT OF OESTRADIOL-17β ON ENZYMES CONCERNED WITH METABOLISM OF CARBOHYDRATE IN HUMAN ENDOMETRIUM IN VITRO

Abstract

SUMMARY: Human endometrium in the regenerative phase was maintained for 5 hr. in vitro with oestradiol-17β alone or together with actinomycin D. Qualitative and quantitative histochemistry of the tissue showed that the activities of glucose-6-phosphate, 6-phosphogluconate, and lactate dehydrogenases were increased by oestradiol, and that actinomycin suppressed the hormonal effect. The activities of succinate and iso-citrate dehydrogenases were unaffected by oestradiol. An attempt is made to correlate the metabolic roles of the enzymes affected by oestradiol. The suppression, by actinomycin, of the oestradiol effect suggests that the increased enzyme activity is due to the formation of new enzyme protein.