Radioiodination studies of tumour cell-surface proteins after different disaggregation procedures
Open Access
- 1 August 1977
- journal article
- research article
- Published by Springer Nature in British Journal of Cancer
- Vol. 36 (2), 166-172
- https://doi.org/10.1038/bjc.1977.173
Abstract
The surface of single cells isolated from solid tumours by either a mechanical or an enzymatic method have been compared, using lactoperoxidase-catalyzed radioiodination of the tyrosine-containing proteins. Qualitatively, the patterns of surface labelling were similar, and duplicate experiments indicated that each method of isolation gave reproducible results. Analysis of incorporated label into 4 defined sections of the electrophoretic pattern illustrated quantitative differences. When the cells were isolated mechanically, the incorporation into low-mol.- wt. components was considerably reduced, whereas that into the high-mol.-wt. components was unaffected. Treatment of enzymatically isolated cells with trypsin also reduced incorporation into low-mol.-wt components.Keywords
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