Identification of 11‐cis and all‐trans‐retinal in the photoreceptive organelle of a flagellate green alga
- 1 November 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 293 (1-2), 49-52
- https://doi.org/10.1016/0014-5793(91)81150-7
Abstract
Isolation of intact photoreceptive organelles (eyespot apparatuses) involved in blue-light mediated photoresponses in a flagellate green alga (Spermatozopsis similis) allowed for the first time the identification of both 11-cis- and all-trans-retinal in a plant cell. Both isomers were identified by HPLC analysis in conjunction with UV spectra. Additionally, reconstitution of a distinct absorption band, centered around 540 nm, was achieved by addition of exogenous 9-cis-retinal to bleached, isolated eyespot apparatuses.Keywords
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