Coadsorption of IgG and BSA onto sulfonated polystyrene latex: I. Sequential and competitive coadsorption isotherms
- 1 January 1996
- journal article
- Published by Taylor & Francis in Journal of Biomaterials Science, Polymer Edition
- Vol. 7 (3), 231-240
- https://doi.org/10.1163/156856295x00274
Abstract
In this work the sequential and competitive coadsorption of IgG and BSA proteins on a sulfonate polystyrene latex with high surface charge density have been studied. For sequential coadsorption the IgG/a-CRP was first adsorbed and then the free surface of the particle was saturated by redispersion of the pellet in a solution with a high concentration of monomeric BSA (m-BSA). The competitive coadsorption experiments were carried out in two separate experiments by changing the initial concentration of one protein when the concentration of other protein was high and constant. During the incubation the pH was 5 or 6, and the ionic strength 2 mM, as in previous studies the adsorption of BSA was very low at neutral or basic pH regardless of the amount of adsorbed IgG. From these coadsorption experiments it was possible to obtain latex-protein complexes with a similar degree of coverage by each protein, high adsorption of IgG and different amounts of BSA, or high adsorption of BSA and a low, but significant, am...Keywords
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