Purification of Glyoxysomal Acetyl-CoA Acyltransferase

Abstract

Acetyl-CoA acyltransferase was extracted from cotyledons of germinating cucumber [Cucumis sativus] seeds and purified to apparent homogeneity. The purification was based primarily on cation exchange chromatography and separation on an affinity gel. The enzyme is a dimer consisting of 2 subunits of MW 45,000. Acetyl-CoA acyltransferase was localized in glyoxysomes, the matrix being the site of thiolase function within the organelle.