Identification and quantitation of cholanoic acids in hepatic and extra‐hepatic tissues of rat
- 1 June 1975
- Vol. 10 (6), 340-347
- https://doi.org/10.1007/bf02532456
Abstract
Tissues of rats were examined for the presence of cholanoic acids. Quantitation of extraction, deconjugation, and isolation were verified by use of radioactive standards. Identification was made by thin layer and gas liquid chromatographic comparison to standards and mass spectrometry. All tissues examined were found to contain several conjugated cholanoic acids. Liver contained primarily cholic acid and peripheral tissues primarily dihydroxy compounds, mainly hyodeoxycholic acid.Keywords
This publication has 15 references indexed in Scilit:
- Cholanoic (bile) acids in hepatic and nonhepatic tissues of miniature swineLipids, 1974
- Metabolism of steroid and amino acid moieties of conjugated bile acids in manJCI Insight, 1972
- Studies on bile acids: The microquantitative separation of cellular bile acids by gas-liquid chromatographyBiochemical Journal, 1967
- Transfer of Radioactivity of Cholesterol-7α-3H to Fatty Acids of Tissue Lipids in vivoNature, 1965
- Gas-liquid chromatography of bile acidsJournal of Oil & Fat Industries, 1965
- Use of a Mass Spectrometer as a Detector and Analyzer for Effluent Emerging from High Temperature Gas Liquid Chromatography Columns.Analytical Chemistry, 1964
- SEPARATION OF STEROIDS BY GAS CHROMATOGRAPHYJournal of the American Chemical Society, 1960
- A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUESJournal of Biological Chemistry, 1957
- A SIMPLIFIED METHOD FOR THE ESTIMATION OF TOTAL CHOLESTEROL IN SERUM AND DEMONSTRATION OF ITS SPECIFICITYJournal of Biological Chemistry, 1952