Recombinant Expression and Characterization of Dentin Matrix Protein 1
- 1 January 1999
- journal article
- research article
- Published by Taylor & Francis in Connective Tissue Research
- Vol. 40 (4), 251-258
- https://doi.org/10.3109/03008209909000703
Abstract
Dentin matrix protein 1 (DMP1) is an extracellular matrix noncollagenous protein (NCP) initially isolated from dentin and now found to be present in calcified tissues like calvaria and long bone. The characteristic feature of DMP1 is that it contains a large number of acidic domains and has properties which implicate it as a key participant in regulating matrix mineralization. The level of DMP1 in the tissue is sparse and it is not easily isolated from dentin because it copurifies with other dentin NCPs. The exact function of DMP1 is not known and this is due to the inherent difficulty of obtaining enough protein from the mineralized tissues. In order to understand the physiologic role for DMP1 during the formation of mineralized tissues we have produced milligram quantities of recombinant DMP1 in E. coli. The objective of this work was: (1) to prepare unmodified apoprotein so that it could be used for studying the function of DMP1; and (2) to prepare polyclonal antibody against the recombinant DMP1 antigen. The DMP1 polyclonal antibody did not cross-react with other NCPs present in dentin or with bone acidic glycoprotein-75 (BAG-75) present in the bone matrix, confirming the specificity of this antibody and thus making it a valuable tool to determine the in vivo function of DMP1.Keywords
This publication has 16 references indexed in Scilit:
- Gene Expression Patterns of Murine Dentin Matrix Protein 1 (Dmp1) and Dentin Sialophosphoprotein (DSPP) Suggest Distinct Developmental Functions In VivoJournal of Bone and Mineral Research, 1997
- Elucidation of the Sequence and the Genomic Organization of the Human Dentin Matrix Acidic Phosphoprotein 1 (DMP1) Gene: Exclusion of the Locus from a Causative Role in the Pathogenesis of Dentinogenesis Imperfecta Type IIGenomics, 1997
- Measurement of antibody/antigen association rate constants in solution by a method based on the enzyme-linked immunosorbent assayJournal of Immunological Methods, 1997
- In SituHybridization Shows Dmp1 (AG1) to Be a Developmentally Regulated Dentin-Specific Protein Produced by Mature OdontoblastsConnective Tissue Research, 1995
- In situ localization and chromosomal mapping of the AG1 (Dmp1) gene.Journal of Histochemistry & Cytochemistry, 1994
- A Glutathione S‐Transferase Fusion Protein with the Starch‐Binding Domain of Aspergillus GlucoamylaseAnnals of the New York Academy of Sciences, 1994
- In vitro Phosphorylation of Mouse Osteopontin Expressed in E. coliBiochemical and Biophysical Research Communications, 1993
- Expression cloning of a cDNA encoding a retinoblastoma-binding protein with E2F-like propertiesCell, 1992
- Bovine dentin phosphophoryn: composition and molecular weightBiochemistry, 1983
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979