Vanadate stimulates tyrosine phosphorylation of two proteins in Raji human lymphoblastoid cell membranes
- 19 September 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 161 (2), 180-184
- https://doi.org/10.1016/0014-5793(83)81003-5
Abstract
A membrane fraction from Raji human lymphoblastoid cells exhibited tyrosine-specific kinase activity. Vanadate increased tyrosine phosphorylation up to 5-fold; serine and threonine phosphorylation were unchanged. The stimulation was detectable within 15 s at 0°C and at concentrations of vanadate (0.3 and 1.0 μM) present in normal tissues and blood. The tyrosine phosphorylation of two substrates, M 1 61 000 and 55 000, was dependent upon vanadate and incorporation into these substrates represented the majority of the vanadate-sensitive tyrosine phosphorylation.Keywords
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