Timing and Precision of Spike Initiation in Layer V Pyramidal Cells of the Rat Somatosensory Cortex

Abstract

The timing and location of action potential initiation in layer V pyramidal cells of the rat somatosensory cortex depends on different ligand-gated and intrinsic conductances. In order to assess the shunting effect of GABA_A receptor activation and I_h on the summative properties of these neurons, we recorded simultaneously from their somata and dendrites and induced combined excitatory and inhibitory postsynaptic potentials using extracellular stimulation. Three major consequences of GABA_A receptor activation on the integration properties were found: (i) the action potential could be initiated in the distal apical dendrite, if GABA_A receptors were simultaneously activated in the soma; (ii) GABA_A receptor activation narrowed the time window for suprathreshold summation of two coincident excitatory synaptic inputs by a factor of 3.4; and (iii) uneven weights of the GABAergic components between two inputs could lead to unbalanced shunting and consequently to a time shift in the peak of the coincidence detection window. Blockade of I_h practically abolished coincidence detection within a time window of 30 ms. Location of spike initiation and summation of coincident inputs in pyramidal cells is determined by at least two shunting mechanisms: activation of the presynaptic inhibitory network and modulation of I_h as a function of the postsynaptic membrane potential.