Correction of CD18-Deficient Lymphocytes by Retrovirus-Mediated Gene Transfer
- 15 June 1990
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 248 (4961), 1413-1416
- https://doi.org/10.1126/science.1972597
Abstract
Leukocyte adhesion deficiency (LAD) is an inherited disorder of leukocyte function caused by derangements in CD18 expression. The genetic and functional abnormalities in a lymphocyte cell line from a patient with LAD have been corrected by retrovirus-mediated transduction of a functional CD18 gene. Lymphocytes from patients with LAD were exposed to CD18-expressing retrovirus and enriched for cells that express CD11a and CD18 (LFA-1) on the cell surface. Molecular and functional analyses of these cells revealed (i) one copy of proviral sequence per cell, (ii) viral-directed CD18 RNA that exceeded normal endogenous levels, (iii) normal quantities of CD11a and CD18 protein on the cell surface, and (iv) reconstitution of LFA-1-dependent adhesive function.This publication has 35 references indexed in Scilit:
- The molecular control of cell division, differentiation commitment and maturation in haemopoietic cellsNature, 1989
- Engraftment of Immune-Deficient Mice with Human Hematopoietic Stem CellsScience, 1988
- Stimulated mobilization of monocyte Mac-1 and p150,95 adhesion proteins from an intracellular vesicular compartment to the cell surface.JCI Insight, 1987
- Heterogeneous mutations in the β subunit common to the LFA-1, Mac-1, and p150,95 glycoproteins cause leukocyte adhesion deficiencyCell, 1987
- Leukocytes from four patients with complete or partial Leu-CAM deficiency contain the common beta-subunit precursor and beta-subunit messenger RNA.JCI Insight, 1987
- Cloning of the $beta; subunit of the leukocyte adhesion proteins: Homology to an extracellular matrix receptor defines a novel supergene familyCell, 1987
- Chromosomal position or virus mutation permits retrovirus expression in embryonal carcinoma cellsCell, 1986
- Deficiency of a leukocyte surface glycoprotein (LFA-1) in two patients with Mo1 deficiency. Effects of cell activation on Mo1/LFA-1 surface expression in normal and deficient leukocytes.JCI Insight, 1984
- Deficiency of a surface membrane glycoprotein (Mo1) in man.JCI Insight, 1984
- Mo2: Functional properties of antigen-bearing cellsClinical Immunology and Immunopathology, 1983