The Isolation and Properties of Some Soluble Proteins From Wool I. The Isolation of a Low-Sulphur Protein

Abstract

Wool was extracted with O� 1M potassium thioglycollate at pH 11� 0 for 2 hr at 50�C and the soluble proteins were precipitated by acidifying to pH 5, redis� solved in potassium thioglycollate at pH 10, and reacted with sodium iodoacetate at pH 9 to give a mixture of at least five electrophoretic ally distinct S�carboxy. methyl kerateines.