Purification of a lipoxygenase from ungerminated barley Characterization and product formation
- 11 March 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 280 (1), 159-162
- https://doi.org/10.1016/0014-5793(91)80227-t
Abstract
Lipoxygenase was purified from ungerminated barley (variety ‘Triumph’), yielding an active enzyme with a pl of 5.2 and a molecular mass of approximately 90 kDa. In addition to the 90 kDa band SDS-PAGE showed the presence of two further proteins of 63 kDa. Western blot analysis showed cross-reactivity of each of these proteins with polyclonal antisera against lipoxygenases from pea as well as from soybean, suggesting a close immunological relationship. he 63 kDa proteins appear to be inactive degradation products of the active 90-kDa enzyme. This barley lipoxygenase converts linolcic acid mainly into (9S)-(10E,12Z)-9-hydroperoxy-10, 12-octadecadienoic acid, and arachidonic acid into (5S)-(6E,8Z,11Z,14Z)-5-hydroperoxy-6,8,11,14-cicosatetraenoic acid.Keywords
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