Production and Characterization of Anti-Murine Interferon-Gamma Sera

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Abstract
A partially purified phytohaemagglutinin (PHA)-induced spleen cell murine interferongamma (MuIFN-γ) proved a potent immunogen in a rabbit and rats. Neutralizing antiMuIFN-γ activity was detected in rabbit serum following two biweekly subcutaneous inoculations of MuIFN-γ emulsified in Freund's complete adjuvant. Further biweekly injections of immunogen in Freund's incomplete adjuvant, greatly elevated the serum antiMuIFN-γ neutralizing titer. Inbred DA rats also responded with serum neutralizing antibodies for MuIFN-γ. Rabbit and rat anti-MuIFN-γ sera neutralized the antiviral activities of MuIFN-γ preparations produced by T-cells in response to different polyclonal T-cell mitogens or by sensitized T-cells stimulated with specific antigen(s). In addition, the antiviral activity of a rat IFN-γ on murine cells was neutralized by rabbit anti-MuIFN-γ serum, but not by the rat anti-MuIFN-γ serum.