Calcium ionophore, phorbol ester, and chemotactic peptide-induced cytoskeleton reorganization in human neutrophils.
Open Access
- 1 May 1987
- journal article
- research article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 79 (5), 1359-1364
- https://doi.org/10.1172/jci112962
Abstract
Formyl-methionylleucylphenylalanine (fMLP) activation of neutrophils causes an increase in intracellular Ca2+, activation of protein kinase C and an increase in F-actin content. To examine the role of Ca2+ and protein kinase C activation as determinants of change in F-actin content of neutrophils, we used the NBD-phallacidin extraction assay to compare the kinetics and extent of change in F-actin content of cells activated with fMLP, the calcium ionophore A23187 or phorbol myristate acetate (PMA). All stimuli increase the F-actin content in a dose-dependent manner; however, the rate of increase is slower and the maximum F-actin content is less for calcium ionophore and PMA than for fMLP-activated cells. The A23187-induced increase in F-actin content, but not that of fMLP, depends upon external free [Ca2+]. In A23187-activated cells, F-actin content increases at [Ca2+]free greater than or equal to 5 microM, is maximal at [Ca2+]free greater than or equal to 10 microM and is negligible at physiologic free [Ca2+] (10(-7)-10(-6) M). Combinations of PMA with A23187 or fMLP inhibit the A23187, but not the fMLP, activated actin polymerization. Comparison and combination of these activators shows that neither Ca2+-dependent activation with A23187 nor activation with PMA alone or in combination mimic the fMLP-induced changes in cytoskeleton organization of neutrophils.This publication has 30 references indexed in Scilit:
- Relationship of actin polymerization and depolymerization to light scattering in human neutrophils: dependence on receptor occupancy and intracellular Ca++.The Journal of cell biology, 1985
- Effects of chemotactic factors and other agents on the amounts of actin and a 65,000-mol-wt protein associated with the cytoskeleton of rabbit and human neutrophils.The Journal of cell biology, 1985
- A Method for quantifying F‐Actin in chemotactic peptide activated neutrophils: Study of the effect of tBOC peptideCell Motility, 1985
- Synergism between phorbol ester and A23187 in Superoxide production by neutrophilsFEBS Letters, 1984
- Superoxide release by neutrophils: Synergistic effects of a phorbol ester and a calcium ionophoreBiochemical and Biophysical Research Communications, 1984
- Chemotactic factor causes rapid decreases in phosphatidylinositol,4,5-bisphosphate and phosphatidylinositol 4-monophosphate in rabbit neutrophilsBiochemical and Biophysical Research Communications, 1983
- Phospholipid-sensitive Ca2+-dependent protein kinase and its substrates in human neutrophilsBiochemical and Biophysical Research Communications, 1983
- Endogenous phospholipid metabolism in stimulated neutrophils differential activation by FMLP and PMABiochemical and Biophysical Research Communications, 1982
- Identification of gelsolin, a Ca2+-dependent regulatory protein of actin gel-sol transformation, and its intracellular distribution in a variety of cells and tissues.The Journal of cell biology, 1981
- The G - F equilibrium in actin solutions under various conditionsBiochimica et Biophysica Acta, 1962