Stomatal Opening Is Induced in Epidermal Peels of Commelina communis L. by GTP Analogs or Pertussis Toxin

Abstract
Pretreatment with pertussis toxin or microinjection of guanosine-5[prime]-(3-thiotriphosphate) (GTP-[gamma]-S) into guard cells in peeled epidermis of Commelina communis L. promoted stomatal opening under subsaturating white light. Guanosine-5[prime]-(2-thiodiphosphate) (GDP-[beta]-S) and adenosine-5[prime]-(3-thiotriphosphate) (ATP-[gamma]-S) did not change stomatal aperture under identical conditions. These results indicate that G proteins may be involved in the regulation of stomatal opening.