A PHOTOELECTRIC METHOD FOR THE QUANTITATIVE DETERMINATION OF ERYTHROCYTE FRAGILITY
Open Access
- 1 September 1940
- journal article
- research article
- Published by American Society for Clinical Investigation in Journal of Clinical Investigation
- Vol. 19 (5), 691-694
- https://doi.org/10.1172/jci101172
Abstract
Photoelectric detn. of the fragility of erythrocytes can be made by placing 0.05 c.c. of oxalated blood in 10 c.c. of hypotonic NaCl solns. of varying conc. ranging from 0.70 to 0.20%. The un-hemqlyzed cells are then thrown down by centrifuging and the liberated oxyhemoglobin detd. photoelectrically. Maximal hemolysis is produced by laking 0.05 c.c. of the same blood in 10 c.c. of 0.1% Na2CO3, and the % hemolysis in varying conc. of NaCl is then expressed in terms of total hemolysis. The curves resulting from plotting % hemolysis against conc. of NaCl are sigmoid in type, and represent the first integral of the probability-distr. of erythrocyte fragility.This publication has 1 reference indexed in Scilit:
- STUDIES ON RED CELL DIAMETERJournal of Clinical Investigation, 1926