Size Exclusion Chromatography of Poly(vinylpyrrolidone)
- 1 May 1987
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 10 (6), 1127-1150
- https://doi.org/10.1080/01483918708066758
Abstract
Poly(vinylpyrrolidone) and poly(ethylene oxide) separate by hydrodynamic volume on Toyo Soda TSK-PW columns in a mixed solvent mobile phase of 50:50 (v/v) MeOH/H2O containing 0.1M LiNO3. From this separation a single universal calibration curve based on hydrodynamic volume [η]M can be obtained. Accurate weight average molecular weights of PVP were obtained by both SEC/LALLS and universal calibration showing good agreement between the two methods. SEC/LALLS overestimates the number average molecular weight for broad distribution polymers due largely to the lack of sensitivity of the LALLS detector to the low molecular weight portion of the polymers, while the universal calibration method slightly underestimates the number average molecular weight as compared to osmometric values.Keywords
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