Radioimmunoassay and intramural distribution of PHI-IR in human intestine
- 31 May 1983
- journal article
- research article
- Published by Springer Nature in Digestive Diseases and Sciences
- Vol. 28 (6), 507-512
- https://doi.org/10.1007/bf01308152
Abstract
The objective of this study was to develop a radioimmunoassay for PHI and use this to assess its intramural distribution in the human intestine. The antibody was harvested following immunization with porcine PHI conjugated to bovine serum albumin by glutaraldehyde, and the iodinated PHI tracer was prepared by the Iodo-gen method. The assay system showed no cross-reaction with other members of the glucagon-secretin family of peptides and was sensitive to changes of PHI of 2 fmol/tube (95% confidence). High concentrations of immunoreactive PHI were found in the human intestine, exclusively localized in the nonendocrine gut layers, suggesting a possible neuroendocrinological or neurotransmitter role for PHI.This publication has 12 references indexed in Scilit:
- Tissue localization and relative distribution of regulatory peptides in separated layers from the human bowelGastroenterology, 1983
- Actions of a newly isolated intestinal peptide PHI on pancreatic aciniAmerican Journal of Physiology-Gastrointestinal and Liver Physiology, 1981
- Isolation and characterization of the intestinal peptide porcine PHI (PHI-27), a new member of the glucagon--secretin family.Proceedings of the National Academy of Sciences, 1981
- Actions of a new peptide from porcine intestine (PHI) on pancreatic secretion in the rat and turkeyLife Sciences, 1980
- Porcine peptide having N‐terminal histidine and C‐terminal isoleucine amide (PHI)FEBS Letters, 1980
- Cyclic AMP production in isolated colonic epithelial crypts: a highly sensitive model for the evaluation of vasoactive intestinal peptide action in human intestineEuropean Journal of Clinical Investigation, 1980
- Interaction of a newly isolated intestinal polypeptide (PHI) with glucose and arginine to effect the secretion of insulin and glucagonLife Sciences, 1980
- Measurement of fasting and postprandial plasma VIP in man.Gut, 1978
- Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphenylglycolurilBiochemical and Biophysical Research Communications, 1978
- Production and Evaluation of Antibodies for the Radioimmunoassay of GastrinScandinavian Journal of Clinical and Laboratory Investigation, 1972