Culture of Apple Shoots from Buds in Vitro1,2

Abstract

Axillary buds from apple shoots when cultured aseptically in vitro readily develop into new shoots. A nutrient medium is used consisting of sucrose and macro and micro elements. Buds grown in stationary culture develop into shoots with small leaves and short internodes. The miniature condition appears to be due to inability of the rootless explants to absorb sufficient nutrients. When buds are cultured in rotating culture where the developing leaves are bathed in nutrient solution, shoots of near normal size and appearance are produced. Light is essential for growth. Low concn of abscisic acid prevents growth of the buds.