Effect of temperature cycling on the production of aflatoxin by Asperfillus parasiticus

Abstract

Aspergillus parasiticus (NRRL 2999) was grown under cycling temperature conditions on rice and nutmeat substrates. Under conditions of diurnal and nocturnal time-temperature sequencing, total heat input is an important factor of toxin production. When expressed in degree hours per day, thermal input becomes more definitive and provides a finite number, which can be related to observable changes in the culture such as sporulation and toxin biosynthesis. Three well-defined levels of response were observed in relation to heat input: no growth was detected at thermal inputs of less than 208 degree hours/day; mycelial growth as well as copious amounts of an orange pigment were observed at thermal inputs between 208 and 270 degree hours/day; sporulation and aflatoxin biosynthesis occurred above 270 degree hours/day. Between the optimum and minimum thermal input, cycling temperatures significantly reduced the period of the trophophase over cultures receiving equal heat input at a constant rate. Cycling temperatures at the low and high extremes of the temperature range had little or no effect upon the growth pattern of the culture. Regardless of how temperature was manipulated, these responses were consistent with the heat input received by the culture. A. parasiticus did not compete well when mixed with natural fungal isolates from nutmeats and was easily overgrown by the wild isolates even at relatively high thermal input and when present in superior numbers. This factor and heat input generally below that required for toxin biogenesis at harvest time appear to be two significant factors that limit occurrence of aflatoxin on nut crops of the Willamette Valley. These factors are likely to have significance for other crops grown and harvested under similar circumstances.