Development and Properties of a Wax Ester Hydrolase in the Cotyledons of Jojoba Seedlings
Open Access
- 1 March 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 61 (3), 339-341
- https://doi.org/10.1104/pp.61.3.339
Abstract
The activity of a wax ester hydrolase in the cotyledons of jojoba (Simmondsia chinensis) seedlings increased drastically during germination, parallel to the development of the gluconeogenic process. The enzyme at its peak of development was obtained in association with the wax body membrane, and its properties were studied. It had an optimal activity at alkaline pH (8.5-9). The apparent Km value for N-methylindoxylmyristate was 93 μM. It was stable at 40 C for 30 min but was inactivated at higher temperature. Various divalent cations and ethylenediaminetetraacetate had little effect on the activity. p-Chloromercuribenzoate was a strong inhibitor of the enzyme activity, and its effect was reversed by subsequent addition of dithiothreitol. It had a broad substrate specificity with highest activities on monoglycerides, wax esters, and the native substrate (jojoba wax).This publication has 7 references indexed in Scilit:
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