Studies on the molecular basis of H+ translocation by cytochromec oxidase
- 1 December 1981
- journal article
- research article
- Published by Springer Nature in Journal of Bioenergetics and Biomembranes
- Vol. 13 (5-6), 219-228
- https://doi.org/10.1007/bf00743201
Abstract
We report here studies which characterize further the interaction ofN,N′-dicyclohexylcarbodiimide with cytochromec oxidase leading to inhibition of H+ translocation by the enzyme. Further evidence is presented to show that the inhibition results from a real interaction of DCCD with the enzyme and cannot be accounted for by uncoupling and, contrary to recent criticisms, this interaction occurs specifically with subunit III of the enzyme even at relatively high inhibitor-to-enzyme stoichiometries. Use of a spin-label analogue of DCCD has enabled us to demonstrate that the carbodiimide-binding site is highly apolar and may not lie on the pathway of electron transfer.Keywords
This publication has 22 references indexed in Scilit:
- Heme aa3-type cytochrome c oxidases from bacteriaBiochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, 1980
- The H+/e− stoicheiometry of respiration-linked proton translocation in the cytochrome system of mitochondriaBiochemical Journal, 1980
- The mechanism of transmembrane ΔμH+ generation in mitochondria by cytochrome c oxidaseBiochemical Journal, 1979
- Limited-turnover studies on proton translocation in reconstituted cytochrome c oxidase-containing vesiclesBiochemical Journal, 1979
- Proton-translocating cytochrome c oxidase in artificial phospholipid vesiclesBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1978
- The Proton Pump of Cytochrome c Oxidase and Its StoichiometryEuropean Journal of Biochemistry, 1978
- Measurements of mitochondrial ←H+/O quotients: Effects of phosphate and N‐ethylmaleimideFEBS Letters, 1978
- Cytochrome c oxidase is not a proton pumpFEBS Letters, 1978
- Studies on CytochromecOxidase, II. The Chemical Constitution of a Short Polypeptide from the Beef Heart EnzymeHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1978
- Site-directed Spin Labeling of the Mitochondrial MembranePublished by Elsevier ,1973