PDF file, 56KB, Fig. S3. Effect of P2X7 inhibition on B16 melanoma growth. Panel A, comparison of basal P2X7 expression in HEK293-mock, CT26wt and B16 tumor cell lines. Cell protein was extracted, run on SDS PAGE, blotted and stained with a cross-reactive human and mouse P2X7-selective polyclonal Ab. Panel B, size of B16 tumor masses injected with 300 nM AZ10606120 in 100 μl of sterile water, or just sterile water, and excised at d 15 (n=7); * p <0.05 versus control B16.