Über das Auftreten von p-Hydroxyphenyl-brenztraubensäure und anderen α-Ketosäuren im Harn von gesunden und leberkranken Kaninchen

Abstract

Liver cirrhosis was induced by intravenous injection of colloidal silicic acid. The urines of these animals were compared with those of a normal control group as to their content of alpha-ketoacids. The acids were precipitated as their hydrozones which were 1st purified according to standard procedure and then reduced to the corresponding amino acids by means of Sn and HC1. The amino acids were adsorbed to Amberlite IR-120, eluted with [image] NH3, and then separated by 2-dimensional paper chromatography. Both normal animals and those with cirrhosis yielded amino acids corresponding to the following alpha-keto acids: glyoxylic, pyruvic, hydroxy-pyruvic, alpha-keto-[image]-butyric, alpha-keto-gamma-methylmercapto-butyric, alpha-keto-beta-methylvaleric, alpha-keto-isocaproic, alpha-ketoglu-taric, oxalacetic and p-hydroxyphenylpyruvic acid. The occurrence of the latter was confirmed separately by coupling with diazotised 4-chloro-2-nitroaniline. It occurred in normal animals, and its excretion was not increased following liver cirrhosis.