Inhibition of lactate dehydrogenase ex rabbit muscle by Cibacron Blue 3G-A bound to water-soluble hydroxyethylcellulose

Abstract

The interaction between lactate dehydrogenase and hydroxyethylcellulose - Cibacron Blue 3G-A conjugates was investigated kinetically. These conjugates were obtained by covalent binding of Cibacron Blue to water-soluble hydroxyethylcelluloses, of average relative molecular masses 2.5 . 10⁴ to 11.6 . 10⁴. The polymeric matrix (non-branched β-glucan) was, unlike the branched α-glucans, found to inhibit the action of the dye, irrespective of the molecular mass of this matrix. With increasing amount of the bound dye the interaction between the dye and the enzyme was enhanced.