Colchicine-Inhibited Cilia Regeneration: Explanation for Lack of Effect in Tris Buffer Medium
- 6 June 1969
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 164 (3884), 1177-1178
- https://doi.org/10.1126/science.164.3884.1177
Abstract
In Stentor coeruleus growth of new, daughter ciliates and experimentaly inducled regeneration of oral membranellar cilia are reversibly inhibited by low, nontoxic concentrations of colchicine. However, if the clulture medium containing colchicine (or Colcemid) is made up in tris(hydroxymethyl)aminomethane buffer, growth of ciliated daughters and regeneration of oral cilia proceed normally. The evidence suggests that the mechanism of this reversal of the effects of colchicine (or Colcemid) is due to a chemical reaction between tris(hydroxymethyl)-aminomethane (or its hydrochloride, or both) and colchicine (or Colcemid), wihich reduces the effective concentration of these mitotic spindle inhibitors reaching the stentors.Keywords
This publication has 7 references indexed in Scilit:
- The Effect of Colchicine on Regenerating Membranellar Cilia in Stentor coeruleusThe Journal of Protozoology, 1969
- PROPERTIES OF THE PROTEIN SUBUNIT OF CENTRAL-PAIR AND OUTER-DOUBLET MICROTUBULES OF SEA URCHIN FLAGELLAThe Journal of cell biology, 1968
- Mitotic Apparatus: The Selective Extraction of Protein with Mild AcidScience, 1968
- Conjugation in Stentor coeruleusThe Journal of Protozoology, 1967
- THE MECHANISM OF ACTION OF COLCHICINEThe Journal of cell biology, 1967
- The biology of StentorPublished by Biodiversity Heritage Library ,1961
- Reactions of Stentor coeruleus to certain substances added to the mediumExperimental Cell Research, 1957